General description

Recombinant, human caspase-5 expressed in E. coli. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.

Recombinant, human caspase-5 expressed in E. coli. A member of the caspase-1 subfamily of cysteine proteases involved in Fas-mediated apoptosis. Caspase-5 cleavage site is reported to be Asp³¹¹ and Asp³³⁰. May also cleave at Asp¹²¹. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.

M.W. 10,000 and 22,000 (heterodimer).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Schwartz, S. et al. 1999. Cancer Res. 59, 2995.Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207.Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.

Packaging

3000 u in Plastic ampoule

Physical form

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

Reconstitution

After initial defrost, aliquot and freeze at -70°C.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805) per min at 25°C, pH 7.4.

Warning

Toxicity: Standard Handling (A)